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To review the principles and contribution of flow cytometry to bacterial analysis, and to show an example of application. Flow cytometry can study many bacterial parameters, such as the membrane potential, membrane integrity, enzymatic activities, some nucleic and antigenic structures, and the level of expression of many genes, using fluorescent reporter systems. Flow cytometry contributed to the analysis of glycopeptide resistance gene expression in Enterococcus faecalis , using a green fluorescent protein GFP as a reporter system.
Flow cytometry is rapid and can provide reproducible, specific and multiparametric analyses on single cells, which may complete the knowledge on structural, physiological and antigenic bacterial properties. Journal page Archives Contents list. Article Article Outline.
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BACTERIOLOGIE MEDICALE (CAMPUS) [Paperback]
Bacteriologie medicale et veterinaire; systematique bacterienne